Effects of Plant Growth Regulators (PGRs) on callus induction from leaf segments explant of Tecomella undulata (Sm.) Seem- An Important Medicinal plant

The present study was conducted to investigate the effects of different concentrations and combinations of plant growth regulators (PGRs) on callus induction of Tecomella undulata (Sm) Seem. The leaf segments were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins such as NAA, 2,4-D, IAA and cytokikinins like BAP and KIN alone and in combinations for callus induction. The maximum callus formation (91.2%) was obtained on MS medium supplemented with in combination BAP with 2,4-D at (3.0+0.5mg/l) and (90.4%) and at (2.5+0.5mg/l) or 2,4-D alone at 3.0 mg/l were the induced calli were whitish green in colour and structurally compact. Remarkable callus biomass of 2.36± 0.06 gm fresh weight and 1.23± 0.08gm dry weight was observed in MS media containing (3.0 mg/l BAP +, 0.5 mg/l 2, 4-D). Abbreviations: MS Murashige and Skoog (1962) basal medium; BAP Benzyl amino purine; KINKinetin 2,4-D 2,4 Dichlorophenoxyacetic acid; NAA a-naphthalene acetic acid; IAA Indole acetic acid.